| In vivo assessment of graphene oxide nanosheets with special emphasis on kidney and brain tissues: Dancing with wolf |
| Paper ID : 1013-ISCH |
| Authors |
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Mohammed Salah Ibrahim *1, Ahmed Said Alazzouni2, Medhat Wahba Shafaa3, Hany Mohamed Hashem3, Magdy Mohamed Khalil4 1Department of Physics, Faculty of Science, Helwan University, Cairo, Egypt. 2Department of zoology, faculty of science, Helwan university, Cairo, Egypt. 3Department of Physics, faculty of science, Helwan university, Cairo, Egypt. 4School of Applied Health Sciences, Badr University in Cairo (BUC), Badr City and Department of Physics, Faculty of Science, Helwan University, Cairo, Egypt. |
| Abstract |
| Background Graphene oxide (GO) is an oxidized form of graphene with different concentrations of sp3 domains and oxygen-containing functional groups such as, hydroxyl, epoxy, and carboxyl groups, which make it a hydrophilic, negatively charged material, and attractive for biomedical applications. Therefore, in the current study, we evaluated the in vivo toxicity of graphene oxide. Methods This study assessed the histological changes associated with intraperitoneally administration of GO into 4 group of mice, namely, deionized water (control group) and various doses of GO (5, 10, 15 mg/kg) every 24 hours for 1 week to receive each animal a total of seven doses. The organs including the kidney and brain were harvested for histopathological evaluations. Results The kidney showed over the three doses showed marked interstial hemorrahgic changes with congested blood vessels, distorted glomeruli with damaged lining epithilium of filtration space, damaged renal capsule and renal tubules as well as degenerative changes of both proximal and distal tubules. The brain tissues displayed over the three doses an increase in the thickness of outer molecular layer of cerebrum with decreased cellularity of outer granular layer. It also showed some cellular vaculations and marked degenrative changes. Moreover, a marked increase of glial cells and decreased neurons with congested blood vessels were also observed. Conclusions The kidney and brain exhibited significant pathological changes following exposure to the three doses. The findings suggest that exposure to the nanographene caused severe damage to both the kidneys and the brain. |
| Keywords |
| Graphene oxide, kidney, and brain. |
| Status: Abstract Accepted (Poster Presentation) |