| Chemopreventive effect of Rice bran extract against AAF-induced hepatocarcinogenesis |
| Paper ID : 1015-ISCH |
| Authors |
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Nermeen S. Okaily *1, Sawsan A. Abd ElHalim2, Hayat M. Sharada3, Gamal S. Essawy4, Eman M. Gouda5 1Biochemistry Division Chemistry Department Faculty of science Helwan university 2Biochemistry Division Chemistry Department Faculty of science Helwan University 3Chemistry Department Faculty of science Helwan university 4Physiology department, Faculty of vetrenary, Cairo University 5Department of Biochemistry and chemistry of nutration , Faculty of vetrenary, Cairo University, Giza, Egypt. |
| Abstract |
| Hepatocarcinoma is a global health concern, due to its high incidence and high rates of mortality. Recently numerous studies suggest that number of nutraceuticals show anticancer properties and may play role in cancer prevention. The present study was conducted to investigate the chemopreventive effect of rice bran extract(RBE) against acetylaminofluorene (AAF) induced rat hepatocarcinogenesis. Forty male albino rats were divided into five groups. AFF control group was given AAF intragastrically three times a week for 12 weeks (3 mg/kg bw in 0.5% carboxymethyl cellulose (CMC). RBE control was orally administered RBE (100 mg/kg bw in 0.5% CMC) daily for 12 weeks. Pre and post RBE groups were treated with RBE at the same dose two week before and after AAF administration for 12 weeks respectively. At the end of experiment DNA was extracted from liver tissues of all rats for SSCP analysis of tumour suppressor gene P53. Serum aspartate transaminase (AST), alanine transaminase (ALT), hepatic malondialdehyde (MDA) and reduced glutathione (GSH) were measured. Pre and post treatments of RBE were decreased the incidence of P53 mutation by 80% and 70% respectively compared to AAF treated group. Both Pre and post treatments with RBE were significantly (P<0.05) decrease levels of liver enzymes AST and AlT as compared to AAF group. Moreover, treatments of RBE before and after AAF administration were significantly (P<0.05) decrease oxidative stress by decreasing hepatic MDA and increasing reduced glutathione as compared to AAF group.this indicated the promising chemopreventive potential of Rice bran. |
| Keywords |
| Hepatocarcinogenesis; Rice bran extract; oxidative stress; P53; chemoprevention |
| Status: Abstract Accepted (Poster Presentation) |