| In vitro antisickling effect of crude and partially purified fractions of methanolic extract of Dichrostachys Cinerea root on human sickled red blood cells |
| Paper ID : 1000-ISCH |
| Authors |
|
Barakat Shuaibu Abdullahi * NSBMB |
| Abstract |
| The in vitro antisickling effect of crude methanolic, ethyl acetate, n-hexane, butanol, and aqueous fractions of Dichrostachys cinerea were analyzed using different concentrations on sodium metabisulphite induced sickled red blood cells. The result showed that except for n-hexane and aqueous fractions, activity was concentration and time dependent, the effect being highest (57.64±4.25, 57.81±5.66, 19.01±5.91, 47.89±4.21, 69.07±0.37,) at (0.2mg/ml 0.2mg/ml 0.2mg/ml 0.3mg/ml 0.2mg/ml) for ethyl acetate, butanol, n-hexane, aqueous, and methanolic fractions respectively. However, in general, for all the fractions, a significant reduction in activity was observed at the highest concentration (0.3mg/ml) suggesting a cytotoxic effect of Dicrostachys cinerea at this concentration. Similarly, all fractions showed a significant difference (p<0.05) when compared with the positive control, parahydroxybenzoic acid. Osmotic fragility test was carried out by challenging erythrocytes in hypotonic saline concentrations of 0.1, 0.2, 0.3, 0.5, 0.6, 0.9ml using extract concentrations of 0.5, 1.0, 2.0, and 2.5mg/ml, n-hexane, butanol, an ethylacetate fractions showed percentage inhibition of heamolysis activity in a proportional manner. A significant difference was observed in percentage inhibition of lysis in the all the test samples when compared with ibuprofen. Percentage methemoglobin concentration was analyzed using extract concentrations of 0.2, 0.4, 0.6, 0.8, and 1mg/ml and a significant difference was observed in the butanol and aqeous and methanolic fractions when compared with the control contrary to the ethylacetate and n-hexane fractions. Elemental analysis showed highest concentrations of Fe, Zn, Cu, and Cr observed in aqueous fraction with a concentration of 287.20±0.00, 72.60±0.00, 57.20±0.00, 20.80±0.00 respectively. |
| Keywords |
| : Antisickling, Dichrostachys cinerea, heamolysis, methemoglobin |
| Status: Abstract Accepted (Oral Presentation) |