Rapid Determination of HMF and Patulin in Commercial Fruit Juices from the Egyptian Market

Paper ID : 1015-ISCH

Authors

Aya Ramadan Mahmoud * Assistant Lecturer's

Abstract

Mycotoxins are toxic secondary metabolites produced by molds, which can affect vertebrates, including mammals, and other animal groups even at very low concentrations. These compounds are of major concern because of their potential to cause acute and chronic toxicity, including carcinogenic, mutagenic, and teratogenic effects. Among these toxins, patulin (PAT) has been widely associated with fruit products, particularly apple-based juices, while 5-hydroxymethylfurfural (HMF) is considered an important quality indicator for processed fruit juices, often formed during heat treatment and storage. In this study, a rapid and simple analytical method was applied for the determination of PAT and HMF in a variety of fruit juices available in the Egyptian market. The method involved extraction of the target compounds using ethyl acetate with vortex-assisted mixing, followed by separation and quantification through high-performance liquid chromatography (HPLC) according to AOAC Official Method 995.10. Results showed HMF concentrations ranged from 0.53 to 477 mg/L, whereas PAT was absent in most samples.

Keywords

HPLC, juice, 5-HMF, patulin

Status: Abstract Accepted (Poster Presentation)