| Ulvan-Mediated Biogenic Selenium Nanoparticles Synergistically Enhance Bone Fracture Healing in a Rat Model |
| Paper ID : 1017-ISCH |
| Authors |
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Ahmed M. Abdelmaguid *1, Sara A. Mekkawy1, Mohamed S. Abdelfattah1, Ahmed E. Abdel Moneim2 1Chemistry Department, Faculty of Science, Helwan University, Cairo, Egypt 2Department of Zoology, Faculty of Science, Helwan University, Cairo, Egypt |
| Abstract |
| This study examined the potential of selenium nanoparticles (SeNPs), which were created using ulvan polysaccharides isolated from Ulva lactuca, to promote fracture healing through osteoinductive, anti-inflammatory, and antioxidant processes. Using a rat model, this work examines the potential therapeutic effects of Ulvan polysaccharide and biogenic SeNPs on bone fracture repair. Rats were given Ulvan, Na₂SeO₃, or Ulvan-SeNPs after being given a standardized mid-diaphyseal femoral fracture. Thorough radiographic, biochemical, and histological analyses were used to gauge fracture healing. According to radiological data, the near-complete obliteration of the fracture line, substantial and well-mineralized callus development, and significant remodeling were signs of superior healing in the Ulvan-SeNPs group. According to biochemical tests, the Ulvan-SeNPs group dramatically decreased inflammatory indicators (NF-κB, IL-1β, TNF-α, and CRP) while increasing antioxidant enzyme activities and bone turnover markers (ALP, osteocalcin, CTX-2). These results were further supported by histological and immunohistochemical analyses, which revealed that the Ulvan-SeNPs group had significant expression of PCNA and TGF-β, minimal fibrous tissue, well-organized new trabecular bone, and robust endochondral ossification. All these findings point to the Ulvan-SeNPs conjugate's considerable promotion of bone regeneration through cellular proliferation and osteogenesis stimulation, antioxidant defense enhancement, and modulation of inflammatory responses. |
| Keywords |
| Bone Fracture Healing, Ulvan, Nanomedicine |
| Status: Abstract Accepted (Oral Presentation) |