Qualitative estimation of some fungal enzymes and its biological activity

Paper ID : 1047-ISCH

Authors

Nada Osama Fathy *

Abstract

L-asparaginase is a crucial therapeutic enzyme vital for treating acute lymphoblastic leukemia by disrupting leukemic cells’ reliance on extracellular L-Asn. Fungal sources have attracted attention as alternatives to bacterial asparaginases due to their high productivity and extracellular secretion. he qualitative estimation of fungal asparaginase is an essential initial step in screening and identifying potential enzyme producers. Commonly, plate assay methods using L-asparagine–supplemented media are employed, where enzyme activity is indicated by a clear zone or pH indicator change around fungal colonies as a result of ammonia release and detecting asparaginase activity in diverse fungal isolates. glutaminase is a microbial enzyme that catalyzes the hydrolysis of L-glutamine to L-glutamic acid and ammonia. It has attracted significant interest due to its biomedical role in cancer therapy, where depletion of L-glutamine restricts tumor cell growth, where enzyme activity is indicated by color change or zone formation around the colonies due to ammonia release. Several genera have demonstrated positive glutaminase . fibrinolytic enzymes are a group of proteolytic enzymes capable of degrading fibrin clots, making them valuable in the treatment of cardiovascular diseases and thrombotic disorders. Microbial production of these enzymes, particularly from fungi, offers advantages such as low production cost, extracellular secretion, and high yield compared to traditional animal-derived fibrinolytic .

Keywords

L-asparaginase, L-glutaminase ,fibrinolytic enzymes, fungi.

Status: Abstract Accepted (Poster Presentation)