| Protective Role of Zeolite Against Cadmium-Induced Testicular Damage Through NF-κB/TNF-α/IL-1β Signaling |
| Paper ID : 1055-ISCH |
| Authors |
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Rania Sayed Ali *1, Asmaa Monir Kandil2, Nehad Mohamed Ibrahim1 1Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo, Egypt 22 Department of Pharmacology, Egyptian Drug Authority, EDA, Formerly NODCAR, Giza, Egypt |
| Abstract |
| Cadmium (Cd) is an environmental pollutant affecting various tissues and organs, including the testis. Many studies demonstrated that Cd toxicity causes testicular impairment through oxidative stress and inflammatory action. Therefore, this study aimed to demonstrate Cd's testicular toxicity and the protective action of zeolite against cadmium's deleterious effects. Adult male rats were given Cd at a dose of 30 mg/kg/day for 28 consecutive days with or without zeolite, which was given at a dose of 100 mg/kg/day for 28 days. Testis weight, sperm (count, motility, and abnormalities), serum testosterone and luteinizing hormone (LH), testicular enzymes Acid phosphatase (ACP) and Alkaline phosphatase(ALP), inflammatory cytokines Tumor Necrosis Factor Alpha (TNF-α) , interleukin-1beta (IL-1β) , and Nuclear Factor Kappa B (NF-κB) and oxidative stress were evaluated. Herein, we found that cadmium caused alterations in sperm characteristics, sex hormone disturbance, decline in testicular enzymes, elevated malondialdehyde (MDA) contents, decreased glutathione (GSH), increased Nuclear Factor Kappa B (NF-κB) and pro-inflammatory cytokines Tumor Necrosis Factor Alpha (TNF-α) and interleukin-1beta ( IL-1β) levels in testis homogenate. In contrast, zeolite significantly amended these deleterious effects, and the potential mechanism involved the downregulation of Nuclear Factor Kappa B (NF-κB), Tumor Necrosis Factor Alpha (TNF-α) and interleukin-1beta (IL-1β), restoring glutathione (GSH ) and reducing malondialdehyde (MDA). Also, zeolite was associated with an increased rate of pregnancy. Our data suggested that oxidative stress and inflammation are responsible for Cd-induced testicular injury and co-administration of zeolite exerts a protective effect via NF-κB /TNF-α/IL-1β pathway. |
| Keywords |
| Cadmium chloride, Zeolite, Testicular injury |
| Status: Abstract Accepted (Poster Presentation) |