| Interaction of Titanium Dioxide, Zinc Oxide and Silver Nanoparticles with Bovine Serum Albumin: Characterization and Biological Significance |
| Paper ID : 1077-ISCH |
| Authors |
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Eman Fekry Abbas *1, Magdy Khalil2, Said Moustafa3, Marwa Abd Ellatif4 1Faculty of science Helwan university 2School of Allied Health Sciences, Badr University in Cairo (BUC), Badr City and Department of Physics, Faculty of Science, Helwan University, Cairo, Egypt. 3Solid state, Department of Physics, Faculty of Science, Helwan University, Cairo, Egypt 4Spectroscopy Department, National Research Center, Dokki, Giza, Egypt. |
| Abstract |
| Abstract Aim. The present study was designed to investigate the interaction mechanism between different types of nanoparticles including titanium dioxide (TiO2 NPs), silver nanoparticles (Ag NPs) and Zinc Oxide (ZnO NPs) versus bovine serum albumin (BSA) by means of multiple spectral methods. Methods. TiO2 NPs, Ag NPs and ZnO NPs were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM). Effect of TiO2 NPs, Ag NPs and ZnO NPs on the conformation of BSA were evaluated by dynamic light scattering (DLS), Fourier-transform infrared (FTIR), UV-Vis, Raman and fluorescence spectroscopies. The cytotoxic effect of nanoparticles and their conjugates with BSA on two different cells –normal and cancer cells- were studied by viability assay. Results. According to the characterization results, the average diameter of TiO2 NPs , Ag NPs and ZnO NPs was approximately 15 nm , 27 nm and 87.7 respectively, making them somewhat spherical particles , Additionally, our findings demonstrated that the size and zeta potential of each NP changed after incubation with BSA. Conclusion: DLS and TEM results showed that interaction of NPs with BSA alters the size and zeta potential of NPs which confirmed the adsorption of BSA on the surface of nanoparticles and the formation of nanoparticle protein corona (NP-PC). FTIR and fluorescence spectroscopy confirmed that NPs-BSA bio-conjugates induced conformational changes in bovine serum albumin and increased proteins helicity. The cytotoxicity results showed that NP-PC reduces the toxicity of NPs. ZnO NPs were the safest nanoparticles on Vero cells and the most toxic nanoparticles on HepG2 cells. |
| Keywords |
| Key words: Titanium nanoparticle, Zinc Oxide, silver nanoparticles, protein corona, bovine serum albumin, binding affinity, nanoparticle characterization. |
| Status: Abstract Accepted (Oral Presentation) |