| Liposomes Bound Antioxidants Concomitantly by Radiation for Cancer Suppression |
| Paper ID : 1079-ISCH |
| Authors |
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Habiba Abdel Moamen Mohamed *1, Medhat Wahba Shafaa2, Eman Ramadan Abbase3, Ahmed Essmet Abdel Moneim4 1Affiliation medical biophysics division, Physics Dept, Faculty of Science, Helwan University 2قسم الفيزياء- كلية العلوم - جامعة حلوان 3قسم الفيزياء- كليه العلوم- جامعه حلوان 4قسم علم الحيوان - كلية العلوم - جامعه حلوان |
| Abstract |
| This study explored the effects of β-carotene delivered in liposomes, with and without polyethylene glycol (PEG) coating, on hepatocellular carcinoma (HepG2) cells in vitro. Prepared liposomes were spherical, well dispersed, and showed slight aggregation. Particle size increased from 110 nm in empty vesicles to 120 nm with β-carotene and 150 nm with PEG. Both FTIR and DSC confirmed strong molecular interactions within the lipid bilayer. Zeta potential measurements revealed improved stability after loading. Cytotoxicity tests showed that free β-carotene was more effective than liposomal formulations under normal conditions, with IC50 values of 12.26 µg/ml for free β-carotene compared to 31.72 µg/ml and 29.78 µg/ml for liposomal and PEGylated forms, respectively. Importantly, combining treatments with gamma irradiation significantly enhanced cytotoxicity, reducing IC50 to as low as 0.57 µg/ml for PEGylated β-carotene. These findings highlight its potential role as a natural radiosensitizer and a promising therapeutic approach for liver cancer, warranting further study. |
| Keywords |
| Keywords: β-carotene; PEGylated liposomes; Gamma irradiation; Hepatocellular carcinoma; Cytotoxicity. |
| Status: Abstract Accepted (Poster Presentation) |