| Clitoria ternatea mitigates cisplatin-induced testicular damage in albino rats |
| Paper ID : 1082-ISCH |
| Authors |
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Khaled Hesham Abdullah *1, Ahmed Said Alazzouni2, Basma Nasr Hassan2, Sherif Rabea Mohamed2, Aya Samir Fathalla2 1Faculty of science-Helwan university 2Faculty of Science, Helwan University |
| Abstract |
| Cisplatin (CIS), a widely used chemotherapeutic agent, is known to cause significant testicular toxicity as a major side effect, leading to impaired fertility in male patients. This study aimed to investigate the protective role of Clitoria ternatea (CT) extract against CIS-induced testicular damage in rats. Forty male rats were randomly divided into four groups (n=10 per group): a control group (Cont.), a cisplatin group (CIS) (7 mg/kg, intraperitoneally), a group receiving CT extract alone (100 mg/kg orally), and group receiving CT extract (100 mg/kg orally) for 21 days following CIS exposure. Key assessments included evaluate biological endpoints, such as testicular weight, relative testes weight, sperm parameters, antioxidant enzyme activity like super oxide dismutase (SOD), Glutathione (GSH), Nitric Oxide (NO), testosterone levels, and histological alterations using specific staining techniques such as hematoxylin and eosin (H&E), Periodic Acid-Schiff stain (PAS) and. along with pronounced degeneration of testicular tissue architecture. Conversely, rats treated with CT extract showed significant improvements in these reproductive parameters, with preservation of seminiferous tubule structure and restoration of hormonal balance. Interestingly, the group that received CT extract alone demonstrated no adverse effects, indicating its safety and potential therapeutic value. These findings suggest that CT flower extract exerts protective effects against CIS-induced testicular toxicity, likely due to its antioxidant, anti-inflammatory, and tissue-regenerative properties. Therefore, CT may offer a promising natural intervention for reducing the reproductive side effects associated with chemotherapeutic agents like CIS. |
| Keywords |
| Infertility, Testicular injury, Sperm count, Oxidative stress, Histopathology |
| Status: Abstract Accepted (Poster Presentation) |