A high-potency probiotic dressing prepared from Lactobacillus plantarum inhibits Staphylococcus aureus and promotes wound healing

Paper ID : 1083-ISCH

Authors

Hazem Sawalha * Professor

Abstract

Wound infections are one of the most prominent medical challenges, especially when healing is delayed by bacterial infection and increasing antibiotic resistance, which impedes the healing process and increases the complexity of treatment. In recent years, probiotic-based dressings, such as Lactobacillus plantarum, have emerged as an innovative option due to their antimicrobial properties and potential ability to support tissue regeneration. This study examines the effectiveness of L. plantarum against Staphylococcus aureus, a major cause of wound infections. The bacteria were isolated from a local fermented yogurt, and their identity was confirmed using multiple laboratory tests. They were then cultured in different media, their supernatants extracted, and bound to gelatin to produce a dressing. Their antibacterial activity was evaluated in a diabetic wound-mimicking medium, and a direct test was performed to confirm their ability to inhibit staphylococcal growth. When the dressing was applied to a superficial wound model in rabbits, the results showed a significant improvement in the healing rate compared to a control wound. Laboratory and in vivo tests also demonstrated that local isolates of L. plantarum retained their antimicrobial capacity, making them a promising option for developing reliable bioactive natural dressings for the treatment of complex wounds.

Keywords

Lactic acid bacteria, Probiotic wound dressing, Antimicrobial resistance, Biofunctional materials

Status: Abstract Accepted (Oral Presentation)