Phytochemical and Biological investigation of Gold Dust leaves

Paper ID : 1114-ISCH

Authors

Shimaa Youssef Mohamed * Masterdegree student

Abstract

Codiaeum variegatum L. (Euphorbiaceae) is an ornamental shrub native to the western Pacific Ocean islands with more than 200 varieties and 300 cultivars among which the compact cultivar, Codiaeum variegatum cv. Gold Dust, showed little literature regarding its chemical and biological significance. Herein, the methanol extract of Codiaeum variegatum cv. Gold Dust leaves and stems was subjected to phytochemical and biological screening. The phytochemical screening revealed the presence of; carbohydrates, flavonoids, tannins, alkaloids, cardiac glycosides, saponins, sterols and/or triterpenes and absence of anthraquinones. Fractionation of the methanol extract with various solvents affords a dichloromethane (DCM), a butanol, an ethyl acetate soluble fractions and a residue. The GC/MS analysis of the DCM content revealed that dodecane is the major identified hydrocarbon (32.13%), while palmitic represents the major identified saturated fatty acid (30.28%). On the other hand, all fractions were compared using high performance liquid chromatography (HPLC) alongside with authentic phenolics. The results identified kaempferol in the four fractions, while, gallic and chlorogenic acids in the ethyl acetate. The caffeic, ferulic, gallic, chlorogenic acids, and rutin were identified in the methanolic residue. According to the knowledge of the traditional uses of the genus Codiaeum susceptibility tests were performed to the four fractions which revealed that the ethyl acetate possesses significant antimicrobial and anti-helicobacter pylori activities, while the butanol fraction showed antimicrobial activity.

Keywords

Gold Dust- HPLC- Anti-Helicobacter pylori

Status: Abstract Accepted (Poster Presentation)